WebRetrieval method: HIER pH6. Protocols. View all protocols. Validation Immunohistochemistry (IHC) All IHC characterization data in 44 normal tissues and 20 cancers for AMAb91409 on the Human Protein Atlas. Target Information Protein Name. T-box 19. Gene Name. TBX19. Alternative Gene Names. dj747L4.1, TPIT, T-PIT. UniProt ID. http://v13.proteinatlas.org/ENSG00000113916-BCL6/antibody
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WebHIER pH6. Concentration. pass. 0.31 mg/ml. Western Blot Score. pass. Band of predicted size in kDa (+/- 20%) with additional bands present ... WebEntdecke Lack Spraydosen Set für Chrysler Dodge Jeep Viper PH6 Light Mahogany Metallic in großer Auswahl Vergleichen Angebote und Preise Online kaufen bei eBay Kostenlose Lieferung für viele Artikel! granberry elem school nashville tn
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WebReagents Required for Heat-induced Epitope Retrieval. 10X Antigen Retrieval Solution: Antigen Retrieval Reagent-Basic (Catalog # CTS013) or Antigen Retrieval Reagent … WebCitrate Buffer Antigen Retrieval Protocol . Description: Formalin or other aldehyde fixation forms protein cross-links that mask the antigenic sites in tissue specimens, thereby giving weak or false negative staining for immunohistochemical detection of certain proteins. The citrate based solution is designed to break the protein cross-links, therefore unmask … The following solutions are three of the more popular buffers for HIER. In the absence of datasheet information, choice of retrieval buffer is best accomplished by trial. Sodium citrate buffer (10 mM Sodium citrate, 0.05% Tween 20, pH 6.0) 1. Tri-sodium citrate (dihydrate) 2.94 g 2. Distilled water 1 L 3. Mix to dissolve. … Ver mais Slides should be placed in a metal rack for this procedure. Materials and reagents 1. Domestic stainless steel pressure cooker 2. Hot plate 3. Vessel with slide rack to hold approximately … Ver mais The enzyme to use will be indicated on the antibody datasheet. If not, trypsin is useful for a wide range of antigens that require retrieval post-formalin/PFA fixation. There are at least two … Ver mais The use of a domestic microwave is inadvisable. Hot and cold spots are common, leading to uneven antigen retrieval. Antigen … Ver mais Many labs use a vegetable steamer or rice cooker for heat-mediated antigen retrieval. The procedure is similar to microwaving in that it maintains the temperature of the buffer at 100°C, but without the vigorous boiling of the … Ver mais granberry funeral home st louis mo