Pb7wgf2
SpletThe primer sequences listed on the left are provided for your reference. Addgene does not distribute primers. For sequencing plasmids in our repository, we've chosen primers … Splet24. maj 2024 · The sequences were mobilized into pBGWFS7, pB7FWG2 or pB7WGF2 (ref. 31) to generate promoter:GFP–GUS reporter lines and p35S promoter driven C- and N-terminal translational GFP fusion constructs ...
Pb7wgf2
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Splet03. avg. 2024 · The pB7WGF2-BPC6 construct was transformed into Agrobacterium tumefaciens strain GV3105 via electroporation. The A. tumefaciens cells that contained pB7WGF2-BPC6 were then introduced into WT. The resulting T1 pB7WGF2-BPC6 transgenic plants were selected by BASTA as described previously (Sun et al., 2016). Homozygous … SpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors
Splet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip … Splet23. nov. 2024 · Agrobacterium tumefaciens GV3101 transformed with pB7WGF2.0 or pK7WGR2.0 harboring each gene of interest or empty (as control) were cultivated overnight at 28 °C in Luria–Bertani medium ...
Splet11. nov. 2015 · Chilling stress is a production constraint of tomato, a tropical origin, chilling-sensitive horticultural crop. The development of chilling tolerant tomato thus has significant potential to impact ... Splet23. jan. 2014 · Next, the AL6 endogenous promoter sequence (1311 bp upstream of the start codon) was PCR-amplified and used to replace the 35S promoter in pB7WGF2:GFP-AL6 by cloning into HindII and SpeI restriction sites, resulting in pAL6:GFP-AL6. pAL6:GFP-AL6 plasmid was introduced into Agrobacterium tumefaciens (GV3101) and the resulting …
Splet11. apr. 2024 · The gaseous hormone ethylene modulates many aspects of plant biology ranging from vegetative development, control of ripening, abscission, senescence, and reproduction to stress responses (1, 2).In most plant tissues, ethylene causes growth reduction due to inhibition of cell expansion, in both roots and shoots (3, 4), acting by …
Splet12. maj 2024 · Pro 35S:GFP-BBX22 lines #1 and #2 were generated in the Col-0 background using a pB7WGF2 vector (Karimi et al., 2002) in which the BBX22 coding DNA sequence (CDS) was inserted after cloning into pDONR221 (primers BBX22_attB1_Fw and BBX22_attB2_Rv, Table S1) using Gateway technology (ThermoFisher Scientific, … marshmallow man backpackSplet26. sep. 2024 · The construct 35Sp-GFP (pB7WGF2 empty vector) was used as a control. These constructs were introduced into Col-0 or jaz4-1 plants according to the floral-dip method for plant transformation (Clough and Bent, 1998). Overexpresser lines were selected by spraying a 0.0114% glufosinate ammonium (BASTA, Bayer®, Whippany, NJ, … marshmallow maker machineSpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors Log In - pB7WGF2 Gateway Vectors Download .Gb File - pB7WGF2 Gateway Vectors Other Resources In this section, clones and vectors co-developed by the Department … For questions related to payments, MTAs, and technical issues, please contact: … The host strain of all plasmids is E. coli DB3.1 or ccdB survival cells from … marshmallow magic cerealhttp://www.biovector.net/product/1684236.html marshmallow man costume boysSplet19. dec. 2024 · A conserved residue in Stphot1, Asp at position 832 (D832), was mutated to Asn (N) generating Stphot1.KD, which results in a kinase-dead version of Stphot1. The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. Agrobacterium … marshmallow man costume babySpletFull Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies … marshmallow man drawingSpletpB7WGF2 plasmid vector植物表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心. 价 格： ¥29865. 货 号： pB7WGF2 plasmid vector植物表达载体质粒. 产 地： 北京. 点击 … marshmallow mallow plant