Thp-1 bcecf protocol
WebMar 1, 2016 · We compare the published protocols using PMA to mature THP-1 cells into macrophages. • The PMA maturation protocol influences the function of the resultant THP-1 macrophages. • THP-1 macrophage functional differences cannot be visualised microscopically. • A standardised protocol for differentiation of THP-1 cells using PMA is … WebMar 18, 2024 · This protocol describes a high-throughput screening assay for the quantitative analysis of cell surface expression of PD-L1 on THP-1 cells. PD-L1 …
Thp-1 bcecf protocol
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http://www.protocol-online.org/biology-forums-2/posts/14943.html WebDec 24, 2024 · An IL-8 Luc test was performed using a THP-1 derived IL-8 reporter cell line THP-G8, ... Osma-Garcia et al. labeled Mφs with BCECF acetoxymethyl ester, and cultured them in plates previously coated with fibronectin ... In Current Protocols in Immunology; Wiley: New York, NY, USA, 2005; Volume Chapter 7 Unit 7.18B. [Google Scholar ...
WebJan 26, 2024 · In this section, we describe the protocol for fixing THP-1 cells (growing in suspension) to the glass coverslip. This is the difficult part for performing confocal microscopy in suspension cells. This protocol is generally useful for other suspension cells. Place the glass coverslip in a 12 well plate, then add 1 mL THP-1 cells (2×10 6 cells). http://www.bowdish.ca/lab/wp-content/uploads/2011/07/THP-1-propagation-culture.pdf
WebJun 20, 2024 · In total, three different protocols were tested differing from the standard protocol as follows: (1) The number of washing steps after primary and secondary antibody staining were reduced from four to three, (2) the aspirated volume was decreased to always leave some liquid in the wells, and (3) the pipetting speed was reduced from 4 to 0.5 µL/s. WebBioz Stars score, Techniques, Protocol Conditions and more for Bcecf Am Labeled Thp 1 Cells, supplied by abcam. Data for Bcecf Am Labeled Thp 1 Cells gathered from related …
WebAn alternate protocol utilizing M-CSF instead of GM-CSF would likely bias generation towards type 2 ... A comparison of differences in the gene expression profiles of phorbol 12-myristate 13-acetate differentiated THP-1 cells and human monocyte-derived macrophage. J Athero Thromb 11(2):88-97, 2004. We are ready to help . Order support;
WebJul 29, 2008 · Although monocytic leukemia cell lines such as THP-1, KG-1, K562, monomac-6, and U-937 had originally been predicted to be good candidates for differentiation into DC-like cells, these leukemia cell lines have been demonstrated to be poor models for DC differentiation, most likely as they are already developed too far along the … eddyville iowa racetrack upcoming eventsWebBioz Stars score, Techniques, Protocol Conditions and more for Bcecf Am Labeled Thp 1 Cells, supplied by abcam. Data for Bcecf Am Labeled Thp 1 Cells gathered from related PubMed articles. Home > Search Results > abcam > … condos on wunderlich for saleWebProtocol status: Working We use this protocol and it's working Created: Mar 13, 2024 Last Modified: Nov 01, 2024 PROTOCOL integer ID: 21416 1 1 ABSTRACT THP-1 cells are a … eddyville ky occupational taxWebTHP-1 cells are a human monocyte suspension cell line from peripheral blood of a 1 year old infant who had acute mnocytic leukemia. eddyville school district oregonWebTHP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter constructs. THP1-Dual™ cells feature the Lucia luciferase gene, ... this protocol can be adapted for use with kinetic measurements or a luminometer with a manual set-up. 1. Add 20 μl of sample per well including Poly(dA:dT) ... eddyville ky to henderson coWebSOP cell culture_THP-1 1.1 3/11 1 Introduction The THP-1 cell line (ATCC: TIB-202) is a human monocytic cell line derived from the peripheral blood of a one year old human male with acute monocytic leukemia (Tsuchiya S. et al., 1980). The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic condos on york north royaltonWebprotocols have been developed treating monocytic cell lines such as U937, HL-60 or THP-1 cells with stimuli such as phorbol-12-myristate-13-acetate (PMA) or 1,25-dihydroxyvitamin D 3 (VD 3) [8,9,10]. The phenotype of the macrophages in these protocols varies with the differentiation treatment and duration, reflecting condos on yacht basin